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. 2011 Sep 26;208(10):1963–1976. doi: 10.1084/jem.20110198

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© 2011 Viatour et al.

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Figure 2. — Inactivation of the Rb gene family in the adult liver results in the expansion of cells with features of stem/progenitor cells. (A) Representative H&E staining (n > 20) of the liver of a cTKO mouse infected with Ad-Cre (4 wk after the injection) shows that TKO early liver lesions are composed of small cells. White dashed lines indicate lesions. All mice used were between 2 and 4 mo of age at the time of injection. PT, portal triad. (B) Sections from TKO liver were stained antibodies against GS (green), a marker of hepatocytes around the central vein (CV). Immunofluorescence images were merged with DAPI (blue) images. The white arrows indicate early TKO lesions. (C) Early lesions in cTKO;Rosa26^LSL-YFP mice infected with Ad-Cre were immunostained for Ki67 and GFP. White dashed lines indicate lesions. (D) Control (CTRL) and TKO liver sections were stained with Sca1 antibodies. The white arrows point to Sca1-positive cells. (E) CTRL and TKO livers were stained with C3, C7, and E10 antibodies. Immunofluorescence images were merged with DAPI images. White arrows indicate early lesions. Bars: (A–D and E, top) 5 µm; (E, bottom) 50 µm.