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. 2011 Sep 26;208(10):2005–2016. doi: 10.1084/jem.20101159

Figure 5.

Figure 5.

Analysis of DCs from WT, Stat1−/−, and IFN-α/βR−/− mice. (A) Wild-type and IFN-α/βR−/− mice were inoculated s.c. with 106 B16.SIY cells. 6 d later, surface expression of CD80, CD86, CD40, and class I and II MHC was assessed by FACS in tumor-draining lymph node cells gated on CD11c+ cells. Filled histograms correspond to an isotype control (IC), continuous line corresponds to wild-type, and dashed line corresponds to IFN-α/βR−/− mice. (B) Adherent splenocytes from wild-type and Stat1−/− mice were loaded with SIY peptide or left untreated and used to stimulate 2C CD8+ T cells. IL-2 production was assessed by ELISA. (C and D) Wild-type Rag1−/− mice (top) or Rag1−/−Stat1−/− mice (bottom) were inoculated with 106 MC57 or MC57.SIY tumor cells. 14 d later SIY/Kb expression was assessed by FACS using high-affinity 2C TCR tetramers gated on the tumor-infiltrating CD11c+ population (C) and CD11b+ population (D). Filled histograms correspond to staining with streptavidin-phycoerythrin alone. Data are representative of two independent experiments (n = 4).