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Artemin mRNA levels. Total mRNA was extracted from the periaqueductal grey matter (PAG) and RT-PCR was performed (Forward primer 5´ TAGGTGGCAACCAGCCTTG 3´; Reverse primer 5´ TGTGTCCCCCAGGTAGGT 3´). Comparison among naive, CCI and CCI-nicotine-treated rats. 1.5mg kg-1 nicotine was administered i.p. for 15 days starting from the day of ligation. Quantitative analysis is shown as the mean ± s.e.m of 5 different animals. *P < 0.01 with respect to naive, saline.
