Venous occlusion of bowel in a Yorkshire pig: Top images: Color images before, during occlusion, and after release, at t = 0, 12, and 20 min, respectively, and over the entire time course. Note a purple discoloration of the tissue during occlusion as blood pools in the tissue. The Vioptix probe can be seen at the bottom right corner of the image. Bottom images: Oxygen saturation images before, during occlusion, and after release, at t = 0, 12, and 20 min respectively, and over the entire time course. Note the significant decrease in saturation during the occlusion. Results are representative of N = 3 separate animals. Top graph: Comparison of SFDI and Vioptix oxygenation measurements as a function of time. SFDI oxygenation saturation values are averaged over a region of interest (black square in SFDI images). Standard deviation of SFDI measurements within the region of interest is also plotted (gray bars). Bottom graph: Concentrations of oxyhemoglobin (ctO2Hb, red) and deoxyhemoglobin (ctHHb, blue) measured using the imaging system as a function of time. Concentration values are averaged over a region of interest (black square in SFDI images). Standard deviation of SFDI measurements within the region of interest is also plotted (gray bars). (Video 2, QuickTime, 0.74 MB).