Figure 5.
Vascular occlusion (arterial and venous) of liver in a Yorkshire pig: Top images: Color images before, during occlusion, and after release, at t = 0, 13, and 20 min, respectively, and over the entire time course. Note a purple discoloration of the tissue during occlusion as oxyhemoglobin is converted to deoxyhemoglobin in the tissue. The Vioptix probe can be seen at the bottom right corner of the image. Bottom images: Oxygen saturation images before, during occlusion and after release, at t = 0, 13, and 20 min, respectively, and over the entire time course. Note the significant decrease in saturation during the occlusion. Results are representative of N = 3 separate animals. Top graph: Comparison of SFDI and Vioptix oxygenation measurements as a function of time. SFDI oxygenation saturation values are averaged over a region of interest (black square in SFDI images). Standard deviation of SFDI measurements within the region of interest is also plotted (gray bars). Bottom graph: Concentrations of oxyhemoglobin (ctO2Hb, red) and deoxyhemoglobin (ctHHb, blue) measured using the imaging system as a function of time. Concentration values are averaged over a region of interest (black square in SFDI images). Standard deviation of SFDI measurements within the region of interest is also plotted (gray bars). (Video 3, QuickTime, 1 MB).