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. Author manuscript; available in PMC: 2011 Sep 28.
Published in final edited form as: Biol Blood Marrow Transplant. 2009 Dec 16;16(3):311–319. doi: 10.1016/j.bbmt.2009.12.002

Figure 4. CCR5 is co-expressed with T cell activation markers.

Figure 4

Mixed lymphocyte culture (MLR) were used to activate PBMC from normal donors with allogenic dendritic cells (DC) mixed with responder PBMCs (DC-MLR) at a DC:PBMC ratio of 1:100. The cells were collected 7 days after stimulation and stained with antibodies CD4, CD8, CCR5 and a panel of activation markers including CD45RA, CD45RO, CD62L and CD69. The representative dot plots of CD4 (upper panel) and CD8 (lower panel) T cells demonstrated the co-expression of CCR5 and activation marks. Data were representative of experiments from three independent samples. Cells were collected on a FACscaliber Flow Cytometer and data was analyzed by Cell Quest software (Becton Dickinson).