Figure 3. Sequence alignment and secondary structure prediction of the ND5 gene for eight species of Oncorhynchus, the NuoL gene for E. coli and the Nqo12 gene from T. thermophilus.

The amino acids in white bold and black background are predicted to be under long-term positive selection in eight species of Oncorhynchus (long-term selection). The amino acids in boxes were determined to have been under positive selection only in O. tshawytscha (episodic selection). Transmembrane domains are shown in bold lettering above gray background-colored amino acids.