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. 2011 Sep 28;6(9):e24127. doi: 10.1371/journal.pone.0024127

Figure 5. Results of the statistical coupling analysis from 428 ND5 amino acid sequences.

Figure 5

The graph of the inverse Inline graphic (1/Inline graphic) shows sites that are ‘unconstrained’ (have a low Inline graphic value or high 1/Inline graphic value) in the ND5 protein compared to a random distribution of amino acids at each site in the MSA (5a). The inverse of Inline graphic is shown for visual simplicity. The y-axis is the 1/Inline graphic and the x-axis is the amino acid position. These could be ‘neutral’ sites or those under positive diversifying selection. The remaining graphs (5b–5h) are the Inline graphic values for each site in the MSA after perturbation for each of the selected sites. Red bars are those sites that are considered significant by TreeSAAP analysis in 15 species of Salmonidae only.