Figure 3. Time-dependent decrease of luminescence intensity of MMP2-GLase with coelenterazine.

Luminescence intensity of MMP2-GLase with coelenterazine (3 µg/ml) is recorded for 60 s. HeLa cells were transfected with pcDNA3-hMMP2-GLuc and cultured for 24 h. After removing the medium, the cells were incubated with HBSS for 60 min at 37°C and the luminescence activity in HBSS was determined using a luminometer, as described in Materials and Methods .