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. 2011 Sep 28;6(9):e25083. doi: 10.1371/journal.pone.0025083

Figure 3. Effects of mSTAT6B and/or mLITAF on mouse CCL2 5′UTR&promoter-enhanced activity.

Figure 3

(a) Diagram of DNA deletions of mouse CCL2 5′UTR&promoter region. The PCR-amplified DNA fragment of mouse CCL2 5′UTR&promoter in the region from −1034∼1, −840∼1, −560∼1, −420∼1, or −280∼1 was respectively inserted into pGL3-basic plasmid as reporter DNAs (named mCL2Pwt, mCL2P840, mCL2P560, mCL2P420, or mCL1P280). (b) Raw264.7 cells were cotransfected with reporter DNAs of mCL2Pwt, mCL2P840, mCL2P560, mCL2P420 or mCL2P280 plus pcDNA3 as control (white bars), mLITAF (pink bars), mSTAT6B (yellow bars) or both mLITAF&mSTAT6B (blue bars) overnight. All test cells were cotransfected with 5 ng of β-gal DNA. The lysate from each test cells was assessed by luciferase assay. Triplicate assays were performed and their values were normalized according to β-gal production and graphed.