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. 2011 Sep 28;6(9):e25083. doi: 10.1371/journal.pone.0025083

Figure 5. Effect of 6BsiRNA1 on LPS-induced serum TNF-α or CCL2 production.

Figure 5

Mice (n = 6×6 groups) were treated as shown in Table 1. Total of 100 ul PBS solution containing 1 mg E. coli LPS plus 40 nM 6BsiRNA2 as control, or plus 20 nM 6BsiRNA1 or 40 nM 6BsiRNA1 was injected into each mouse. Blood was collected 5 hrs after injection. Mice were warmed under heating lamps to promote blood flow, and a small incision was made on the tail. About 50 µl of blood was collected per mouse. Red blood cells were removed from the sample via centrifugation at 5,000 rpm for 1 min using serum separator tubes. Top Panel: protein expression from each sample (Nos 1-7) detected by Western blot using antibodies against mLITAF, 5278 or Actin (control). Lower panel: serum levels of TNF-α (a), CCL2 (b) or IL-1β, IL-6 (c) from each sample measured by ELISA. ELISA immunoreactivity was quantified using a microplate reader and measurements were graphed. The assays were performed in triplicate and a representative experiment is presented.