Table 2.

Mediator	ELISA analysis of gene secretion induced by mLITAF overexpression (+/−)*	BLAST SEARCH of the location for the putative binding sequence, CTCCC.**
TNF-α	+	−16∼−20, −85∼−89, −155∼−159, −633∼−637
MCP-1	+	319∼−315, −298∼−294
MCP-2	+	−85∼−89
IL-1α	+	340∼−344
IL-10	+	−743∼−747, −867∼−871
RANTES	+	−22∼−26, −90∼−94, −138∼−142, −362∼−366
IL-6	−	None
IL-1β	−	None

*Raw264.7 cells were transfected with mLITAF or pcDNA alone as control o/n. The secretion protein in the conditioned medium was assessed by ELISA. 200% increase or more in protein concentration compared to the control was assigned with (+), but it was considered a minus (−) if the protein level was 100% or less than the control.

**Bioinformatic analysis of binding site of TNF-α (U68414), MCP-1 (AL713839), MCP-2 (AL713839), IL-1α (NT_039207.7), IL-10 (HQ014592), RANTES (NT_096135.5), IL-6 (NT_162294.3), or IL-1β (NT_039207.7) in their promoter region from −1 to −900.