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. 2011 Sep 28;6(9):e25313. doi: 10.1371/journal.pone.0025313

Figure 1. Deletion of Tb927.3.1830 causes a growth defect.

Figure 1

(A) Alignment of Tb927.3.1830 with human, mouse and chicken RMI1. DUF1767 and OB-fold domains are indicated in pink and yellow boxes, respectively. Conserved lysine residues are in green box in the OB1 domain. Blue bars show locations of five β-strands of OB1. (B) Deletion scheme for Tb927.3.1830. The entire open reading frames (ORFs) of Tb927.3.1830 were sequentially deleted using cassettes containing HYG-TK or PUR flanked by loxP sites. The markers were removed by transient expression of Cre-recombinase [39], [64]. (C) Tbrmi1 exhibits a minor growth defect, similar to Tbtopo3α. Wild-type, rmi1-/+ and rmi1-/- cells were diluted to 10,000 cells/ml and cells were counted after two days of incubation. This was repeated twice. Growth phenotypes of Tbtopo3α mutants were taken from the previous study [39]. Error bars are shown, but are small. (D) Diagrams of human RMI1/2 [41] and Tb927.3.1830.