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. 2011 Sep 28;6(9):e25313. doi: 10.1371/journal.pone.0025313

Figure 3. TbRMI1 deficiency increases VSG switching frequency.

Figure 3

(A) VSG switching reporter cell line and switching mechanisms. The active expression site, ES1-VSG 427-2, was doubly marked with BSD at the promoter and PUR-TK immediately at the 3′ end of the 70-bp repeats. VSG switching events will accompany either loss or repression of the PUR-TK marker, which allows only the switchers to grow in the presence of GCV [39]. Non-switchers can be eliminated as described in the text. Black circles are telomere repeats. (B) TbRMI1 deficiency increases VSG switching frequency. Switching frequency was measured in wild type, rmi1, and rmi1 cells transfected either with an empty vector or the wild-type RMI1.