Figure 5. Genetic interaction between T. brucei RMI1 and TOPO3α.

(A) TbRMI1 is epistatic to TbTOPO3α in VSG switching. (B) RMI1 absence increases gene-conversion frequency at URA3 locus and TbRMI1 is epistatic to TbTOPO3α in gene conversion at this locus. Two counter-selectable markers, TK and URA3, were used to measure the GC rates [39]. GCV^R and FOA^R clones were counted and fold increase relative to wild type was plotted. Error bars indicate standard deviation. (*) in Figure 5B indicates topo3α data taken from the previous report [39]. Unpaired T tests were done and means of switching and GC frequencies were significantly different in the mutants.