Table 2. Strains used in this study.
Names (sources) | Genotypes | Manipulations |
SM | WT | T7 RNA polymerase and Tet repressor (TetR):: NEO |
HSTB-188 [39] | WT | ES1 promoter::BSD |
HSTB-261 [39] | WT | ES1 promoter::BSD, 70-bp repeats-PUR-TK |
HSTB-229 | rmi1-/+ | ES1 promoter::BSD, rmi1ΔloxP-HYG-TK-loxP/+ |
HSTB-286 | rmi1-/- | ES1 promoter::BSD, rmi1ΔloxP-HYG-TK-loxP/ΔloxP-PUR-loxP |
HSTB-298 | rmi1-/- | ES1 promoter::BSD, rmi1ΔloxP/ΔloxP |
HSTB-336 | rmi1-/- | ES1 promoter::BSD, 70-bp repeats-PUR-TK, rmi1ΔloxP/ΔloxP |
HSTB-411, 412, 413 | rmi1-/- + Vector | ES1 promoter::BSD, 70-bp repeats-PUR-TK, rmi1ΔloxP/ΔloxP + pHD309 (empty vector) |
HSTB-414, 415, 416 | rmi1-/- + RMI1-wt | ES1 promoter::BSD, 70-bp repeats-PUR-TK, rmi1ΔloxP/ΔloxP + pSY38 (RMI1-wt in pHD309) |
HSTB-344 [39] | topo3α-/- | ES1 promoter::BSD, 70-bp repeats-PUR-TK, topo3αΔloxP/ΔloxP |
HSTB-365 [39] | rad51-/- | ES1 promoter::BSD, 70-bp repeats-PUR-TK, rad51ΔHYG/rad51ΔPHELO |
HSTB-384, 385, 386 | rmi1-/- rad51-/- | ES1 promoter::BSD, 70-bp repeats-PUR-TK, rmi1ΔloxP/ΔloxP, rad51ΔHYG/rad51ΔPHELO |
HSTB-456 | rmi1-/- topo3α-/- | ES1 promoter::BSD, 70-bp repeats-PUR-TK, rmi1ΔloxP/ΔloxP, topo3αΔloxP/ΔloxP |
HSTB-201 | 2040-3HA (*) | 2040-3HA::HYG |
HSTB-210 | RMI1-3HA | RMI1-3HA::PUR |
HSTB-213 | TOPO3α-3MYC | TOPO3α-3MYC::PHLEO |
HSTB-218 | 2040-3HA( * ) TOPO3α-3MYC | 2040-3HA::HYG, TOPO3α-3MYC::PHLEO |
HSTB-222 | RMI1-3HA TOPO3α-3MYC | RMI1-3HA::PUR, TOPO3α-3MYC::PHLEO |
(*) The full annotation is Tb927.8.2040.