pHD309 |
Vector to target at TUB array, HYG
|
|
pLHTL-pyrFE |
TbURA3::HYG-TK
|
[64]
|
pLEW100-Cre |
Cre-recombinase in expression vector, PHLEO
|
[64]
|
pHJ17 |
loxP-HYG-TK-loxP
|
[39]
|
pHJ18 |
loxP-PUR-TK-loxP
|
[39]
|
pHJ23 |
To target BSD downstream of ES promoter |
[39]
|
pHJ63 |
TOPO3α Δ::loxP-PUR-TK-loxP
|
[39]
|
pHJ64 |
TOPO3α Δ::loxP-HYG-TK-loxP
|
[39]
|
pSY1 |
RAD51Δ::HYG
|
[39]
|
pSY23 |
RAD51Δ::PHLEO
|
[39]
|
pHJ71 |
RMI1Δ::loxP-HYG-TK-loxP
|
This study |
pHJ77 |
RMI1Δ:: loxP-PUR-loxP
|
This study |
pSY38 |
To insert wild-type RMI1 at TUB array, HYG
|
This study |
pMOTag53M |
One-step PCR-3xMYC tagging construct, PHLEO
|
[42]
|
pMOTag4H |
One-step PCR-3xHA tagging construct, HYG
|
[42]
|
pMOTag2H |
One-step PCR-3xHA tagging construct, PUR
|
[42]
|