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. 2011 Sep 28;6(9):e25313. doi: 10.1371/journal.pone.0025313

Table 3. Plasmids used in this study.

Names Inserts, targeting loci, and markers Sources
pHD309 Vector to target at TUB array, HYG
pLHTL-pyrFE TbURA3::HYG-TK [64]
pLEW100-Cre Cre-recombinase in expression vector, PHLEO [64]
pHJ17 loxP-HYG-TK-loxP [39]
pHJ18 loxP-PUR-TK-loxP [39]
pHJ23 To target BSD downstream of ES promoter [39]
pHJ63 TOPO3α Δ::loxP-PUR-TK-loxP [39]
pHJ64 TOPO3α Δ::loxP-HYG-TK-loxP [39]
pSY1 RAD51Δ::HYG [39]
pSY23 RAD51Δ::PHLEO [39]
pHJ71 RMI1Δ::loxP-HYG-TK-loxP This study
pHJ77 RMI1Δ:: loxP-PUR-loxP This study
pSY38 To insert wild-type RMI1 at TUB array, HYG This study
pMOTag53M One-step PCR-3xMYC tagging construct, PHLEO [42]
pMOTag4H One-step PCR-3xHA tagging construct, HYG [42]
pMOTag2H One-step PCR-3xHA tagging construct, PUR [42]