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. 2011 Sep 28;6(9):e25465. doi: 10.1371/journal.pone.0025465

Figure 5. Periventricular dysplastic mass in mDia-DKO mice consists of heterogeneous cell populations of mitotic neural progenitors and non-mitotic neurons.

Figure 5

(A, B) Neuro-rosettes in periventricular dysplastic mass of mDia-DKO mice. Neuro-rosette cells (arrows in B) are arranged around a central space filled with fibrillar extensions of the cells (B). A large number of cells with condensed chromatin presumably undergoing mitosis surrounded the fibrillar extensions of neuro-rosettes. (A, B) Scale bars, 50 µm. (C, D) H&E staining of coronal brain sections from control mDia3null (C) and mDia-DKO (D) mice at P0. A region of periventricular dysplastic mass is outlined by dotted lines. Note large periventricular dysplastic mass that completely occupies the lateral ventricle in one hemisphere and disrupts the continuity of the ventricular zone/subventricular zone of the lateral ventricle. (C, D) Scale bars, 500 µm. (E) Higher magnification of H&E staining of a coronal brain section of mDia-DKO mouse at P0. periventricular dysplastic mass, a boundary of which is outlined, is composed of heterogeneous cells of different H&E staining patterns. White, black and yellow asteriks (D and E) indicate the cell populations with dense, light nuclear staining and strong eosin staining, respectively. (E) Scale bar, 250 µm. (F) Phospho-histone H3 (PH3) immunoreactivity (green) and Hoechst nuclear staining (red). Mitotic cells are localized in the periventricular dysplastic mass cell population with dense nuclear staining in addition to cells in ventricular zone of lateral wall and basal progenitors. (F) Scale bar, 100 µm. (G–I) Nestin immunoreactivity (green) and Hoechst nuclear staining (red) in periventricular dysplastic mass. Dotted lines show the boundary between periventricular dysplastic mass and the ventricular zone. (J–L) Tuj1 immunoreactivity (green) and Hoechst nuclear staining (red) in periventricular dysplastic mass. Dotted lines encircle the dense nuclear staining cell mass. Cells with dense nuclear staining in the periventricular dysplastic mass were nestin positive, while cells with lighter nuclear staining were Tuj1 positive. (G–L) Scale bars, 100 µm.