Table 1. Bacterial strains used in this work.
Strain | Relevant characteristics | Source/reference |
Yersinia pestis : | ||
CO92 Pgm− | Attenuateda, Pmxr (polymyxin B resistant) | Lab collection [49] |
CO92 Pgm− lpxM | Kmr-cassette replacement of YPO2063 (lpxM)b gene, Pmxr | This work |
CO92 Pgm− yrbH | Kmr-cassette replacement of YPO3577 (yrbH) gene, Pmxs | This work |
CO92 Pgm− yrbH (pBADc) | Transformed with a pBAD vector (Apr, Invitrogen) | This work |
CO92 Pgm− yrbH (pYrbH) | pYrbH is pBAD with cloned yrbH | This work |
CO92 Pgm− waaA | Kmr-cassette replacement of YPO0055 (waaA) gene, Pmxs | This work |
CO92 Pgm− waaA (pBAD) | Transformed with pBAD | This work |
CO92 Pgm− waaA (pWaaA) | pWaaA is pBAD with cloned waaA | This work |
CO92 Pgm− hldE | Kmr-cassette replacement of YPO0654 gene, Pmxs | This work |
CO92 Pgm− hldE (pBAD) | Transformed with pBAD | This work |
CO92 Pgm− hldE (pHldE) | P654 is pBAD with cloned YPO0654 | This work |
CO92 Pgm− waaF | Kmr-cassette replacement of YPO0057 (waaF) gene, Pmxs | This work |
CO92 Pgm− waaF (pBAD) | Transformed with pBAD | This work |
CO92 Pgm− waaF (pWaaF) | pWaaF is pBAD with cloned waaF | This work |
CO92 Pgm− waaL | Kmr-cassette replacement of YPO0417 (waaL) gene, Pmxr | This work |
CO92 Pgm− waaL (pBAD) | Transformed with pBAD | This work |
CO92 Pgm− waaL (pWaaL) | pWaaL is pBAD with cloned waaL | This work |
CO92 Pgm− wabD | Kmr-cassette replacement of YPO0187 gene, Pmxr | This work |
CO92 Pgm− wabC | Kmr-cassette replacement of YPO0186 gene, Pmxr | This work |
CO92 | Fully virulent strain, from human case of pneumonic plague, Pmxr | Lab collection [52] |
CO92 yrbH-1 | Kmr-cassette replacement of YPO3577 (yrbH) gene, Pmxs | This work |
CO92 yrbH-2 | An independent yrbH mutant, Pmxs | This work |
CO92 waaA-1 | Kmr-cassette replacement of YPO0055 (waaA) gene, Pmxs | This work |
CO92 waaA-2 | An independent waaA mutant, Pmxs | This work |
CO92 hldE | Kmr-cassette replacement of YPO0654 gene, Pmxs | This work |
CO92 waaF | Kmr-cassette replacement of YPO0057 (waaF) gene, Pmxs | This work |
CO92 waaL | Kmr-cassette replacement of YPO0417 (waaL) gene, Pmxr | This work |
CO92 S-2 | Spontaneous L-413C-resistant mutant, Pmxs | This work |
CO92 S-7 | Spontaneous L-413C-resistant mutant, Pmxr | This work |
KIM D27 | KIM Pgm−, attenuateda, Pmxr | Lab collection [53] |
A1122 | Pgm− pCD1−, attenuateda, Pmxr | Lab collection [54] |
Yersinia pseudotuberculosis : | ||
PB1 | Serovar IB | Lab collection [55] |
Escherichia coli : | ||
C600 | K-12 derivative; λ– supE44 tonA21 (rk +mk +) | Lab collection [56] |
C600 pUTKm | pUTKmd is a Tn903 kan gene source [57] | Lab collection |
TOP10 | ΔlacX74 deoR recA1 rpsL, endA1, nupG | Invitrogen |
C-520 | “Restrictionless” P2 phage indicator strain | R. Calendare [58] |
These strains are attenuated due to a lack of pigmentation/siderophore yersiniabactin production genes and/or of pCD1 plasmid encoding type III secretion system and virulence Yop proteins (for review, see [1]).
Clones with a pBAD vector (Invitrogen, Carlsbad, California, USA) were used in trans-complementation experiments to exclude a possible impact of pBAD on LPS expression and phage susceptibility.
Plasmid pUTKm was used as a source of kanamycin cassette.
Department of Molecular and Cell Biology, University of California, Berkeley, California, USA.