Skip to main content
. 2011 Sep 28;6(9):e25486. doi: 10.1371/journal.pone.0025486

Table 1. Bacterial strains used in this work.

Strain Relevant characteristics Source/reference
Yersinia pestis :
CO92 Pgm Attenuateda, Pmxr (polymyxin B resistant) Lab collection [49]
CO92 Pgm lpxM Kmr-cassette replacement of YPO2063 (lpxM)b gene, Pmxr This work
CO92 Pgm yrbH Kmr-cassette replacement of YPO3577 (yrbH) gene, Pmxs This work
CO92 Pgm yrbH (pBADc) Transformed with a pBAD vector (Apr, Invitrogen) This work
CO92 Pgm yrbH (pYrbH) pYrbH is pBAD with cloned yrbH This work
CO92 Pgm waaA Kmr-cassette replacement of YPO0055 (waaA) gene, Pmxs This work
CO92 Pgm waaA (pBAD) Transformed with pBAD This work
CO92 Pgm waaA (pWaaA) pWaaA is pBAD with cloned waaA This work
CO92 Pgm hldE Kmr-cassette replacement of YPO0654 gene, Pmxs This work
CO92 Pgm hldE (pBAD) Transformed with pBAD This work
CO92 Pgm hldE (pHldE) P654 is pBAD with cloned YPO0654 This work
CO92 Pgm waaF Kmr-cassette replacement of YPO0057 (waaF) gene, Pmxs This work
CO92 Pgm waaF (pBAD) Transformed with pBAD This work
CO92 Pgm waaF (pWaaF) pWaaF is pBAD with cloned waaF This work
CO92 Pgm waaL Kmr-cassette replacement of YPO0417 (waaL) gene, Pmxr This work
CO92 Pgm waaL (pBAD) Transformed with pBAD This work
CO92 Pgm waaL (pWaaL) pWaaL is pBAD with cloned waaL This work
CO92 Pgm wabD Kmr-cassette replacement of YPO0187 gene, Pmxr This work
CO92 Pgm wabC Kmr-cassette replacement of YPO0186 gene, Pmxr This work
CO92 Fully virulent strain, from human case of pneumonic plague, Pmxr Lab collection [52]
CO92 yrbH-1 Kmr-cassette replacement of YPO3577 (yrbH) gene, Pmxs This work
CO92 yrbH-2 An independent yrbH mutant, Pmxs This work
CO92 waaA-1 Kmr-cassette replacement of YPO0055 (waaA) gene, Pmxs This work
CO92 waaA-2 An independent waaA mutant, Pmxs This work
CO92 hldE Kmr-cassette replacement of YPO0654 gene, Pmxs This work
CO92 waaF Kmr-cassette replacement of YPO0057 (waaF) gene, Pmxs This work
CO92 waaL Kmr-cassette replacement of YPO0417 (waaL) gene, Pmxr This work
CO92 S-2 Spontaneous L-413C-resistant mutant, Pmxs This work
CO92 S-7 Spontaneous L-413C-resistant mutant, Pmxr This work
KIM D27 KIM Pgm, attenuateda, Pmxr Lab collection [53]
A1122 Pgm pCD1, attenuateda, Pmxr Lab collection [54]
Yersinia pseudotuberculosis :
PB1 Serovar IB Lab collection [55]
Escherichia coli :
C600 K-12 derivative; λ– supE44 tonA21 (rk +mk +) Lab collection [56]
C600 pUTKm pUTKmd is a Tn903 kan gene source [57] Lab collection
TOP10 ΔlacX74 deoR recA1 rpsL, endA1, nupG Invitrogen
C-520 “Restrictionless” P2 phage indicator strain R. Calendare [58]
a

These strains are attenuated due to a lack of pigmentation/siderophore yersiniabactin production genes and/or of pCD1 plasmid encoding type III secretion system and virulence Yop proteins (for review, see [1]).

b

Y. pestis CO92 LPS gene designations are given according to [50], [51].

c

Clones with a pBAD vector (Invitrogen, Carlsbad, California, USA) were used in trans-complementation experiments to exclude a possible impact of pBAD on LPS expression and phage susceptibility.

d

Plasmid pUTKm was used as a source of kanamycin cassette.

e

Department of Molecular and Cell Biology, University of California, Berkeley, California, USA.