Table 2. Bacteriophages used in this work.
Bacteriophage | Group | Source | Reference(s) | LPS residues critical for phage receptors (determined in this work) |
L-413C | P2 | Lab collection | [14], [19], [23], [24], [27] | GlcNAc |
P2 vir1 | P2 | R. Calendar | [59] | GlcNAc |
ϕJA1a | P2? | Sewage | This work | Kdo/Ko |
ϕA1122 | T7 | M.E. Schrieferb | [10], [18], [22], [25]–[27] | Kdo/Ko |
T7 | T7 | I.J. Molineuxc | [60] | NAd |
T7Yp e | T7 | T7 host range mutant | This work | HepI/Glc |
Pokrovskaya | NDf | B.B. Atshabarg | [9], [14], [23], [24] | HepII/HepIII |
Y | T3? | ATCC | [12], [13] | HepI/Glc |
PST | T2? | ATCC | [12], [61]–[63] | HepII/HepIII |
Rh | ND | ATCC | [12], [15], [63]–[65] | Beyond LPS core |
Phage ϕJA1 isolated from sewage lyses multiple Y. pestis strains but not Y. pseudotuberculosis or E. coli K-12; it has some degree of homology to L-413C because its DNA is amplified in qPCR with one of two tested pairs of primers specific for L-413C (A.A. Filippov, Y. He, and K.V. Sergueev, unpublished data).
Bacterial Diseases Branch, Division of Vector-Borne Infectious Disease, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, Ft. Collins, Colorado, USA.
Section of Molecular Genetics and Microbiology, and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas, USA.
NA, not applicable.
T7Yp is a T7 host range mutant isolated on the lawn of Y. pestis CO92; T7Yp produces plaques on Y. pestis but not on Y. pseudotuberculosis or Yersinia enterocolitica and has a low plaquing efficiency on E. coli K-12 (A.A. Filippov, Y. He, and K.V. Sergueev, unpublished data).
ND, not determined.
Kazakh Scientific Center for Quarantine and Zoonotic Diseases, Almaty, Kazakhstan.
R phage (R stands for “Russian” [12]) was originally isolated and studied by R.I. Kotlyarova [64] and is also designated as the Kotlyarova phage. Pseudotuberculosis diagnostic phages PST [63] and R [15] can lyse 92–100% of Y. pseudotuberculosis strains but are also active against the majority of Y. pestis strains.