Table 4. Plaquing efficiencies of various phages on Y. pestis CO92 LPS-affected mutants.
Y. pestis straina | Efficiencies of platingb | ||||||||
L-413C | P2 vir1 | ϕJA1 | ϕA1122 | Pokrovskaya | T7Yp | Y | PST | R | |
CO92 Pgm− (parental) | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
lpxM | 1.1 | 0.8 | 0.1 | 0.4 | 1.2 | 1.7 | 1.0 | 1.1 | 1.0 |
yrbH | <2.0×10−12 | <5.0×10−8 | <7.1×10−10 | 1.2×10−4 | 6.9×10−12 | 1.1×10−9 | 5.3×10−9 | <7.1×10−12 | 0.1 |
waaA | <2.0×10−12 | <5.0×10−8 | <7.1×10−10 | 1.4×10−3 | <7.7×10−13 | 1.3×10−7 | 3.0×10−7 | <7.1×10−12 | 20 |
hldE | <2.0×10−12 | <5.0×10−8 | 7.1×10−5 | 0.8 | 1.1×10−3 | 3.3×10−2 | 5.6×10−6 | <7.1×10−12 | 120 |
waaF | 2.8×10−4 | <5.0×10−8 | 3.6×10−5 | 0.6 | 8.5×10−4 | 0.1 | 0.2 | <7.1×10−12 | 60 |
waaL | <2.0×10−12 | <5.0×10−8 | 6.4×10−3 | 0.9 | 0.8 | 1.9 | 1.0 | 1.4 | 300 |
wabD | 0.9 | 0.7 | 1.9 | 1.2 | 1.4 | 1.8 | 0.7 | 1.2 | 90 |
wabC | 1.0 | 1.0 | 3.5 | 1.4 | 2.0 | 0.7 | 1.3 | 0.9 | 210 |
The phage plaquing tests were performed at 28°C.
The titers of bacteriophages (PFU/ml) determined on CO92 Pgm− strain were: 5.0×1011 (L-413C), 2.0×107 (P2 vir1), 1.4×109 (ϕJA1), 5.0×1012 (ϕA1122), 1.3×1012 (Pokrovskaya), 9.0×1011 (T7Yp), 3.0×1011 (Y), 1.4×1011 (PST), and 1.0×109 (R).