Table 5. Restoration of phage susceptibility as a result of trans-complementation of Y. pestis LPS-affected mutants.

Y. pestis straina	Susceptibility tob:
	L-413C	P2 vir1	ϕJA1	ϕA1122	Pokrovskaya	T7Yp	Y	PST
CO92 Pgm− (parental)	+	+	+	+	+	+	+	+
yrbH c	−	−	−	−	−	−	−	−
yrbH (pYrbHd)	+	+	+	+	+	+	+	+
waaA	−	−	−	−	−	−	−	−
waaA (pWaaA)	+	+	+	+	+	+	+	+
hldE	−	−	−	+	−	−	−	−
hldE(pHldE)	+	+	+	+	+	+	+	+
waaF	−	−	−	+	−	+	+	−
waaF (pWaaF)	+	+	+	+	+	+	+	+
waaL	−	−	−	+	+	+	+	+
waaL (pWaaL)	+	+	+	+	+	+	+	+

^aThe phage plaquing tests were performed at 28°C.

^b +, phage-susceptible; −, phage-resistant.

^cEach of the mutants was also transformed with a pBAD vector without insert to exclude possible impact of the vector on LPS structure and phage susceptibility; all pBAD⁺ transformants had the same phenotype as the parental mutant (data not shown).

^dSee characteristics of the recombinant plasmids in Table 1 and Materials and Methods.