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. 2011 Sep 28;6(9):e25650. doi: 10.1371/journal.pone.0025650

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Lackey et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Number and protein content of N. crassa TOB complexes. Mitochondria were isolated from strains expressing only His-tagged versions (instead of the endogenous versions) of either Tob55 (Tob55HT), Tob38 (Tob38HT), or Tob37 (Tob37HT) as indicated for each lane at the top of the panel. TOB complexes were purified using Ni-NTA resin. Purified complexes were subjected to BNGE, transferred to PVDF membrane, and decorated with antibodies to Tob55, Mdm10, Tob37, or Tob38 as indicated at the bottom of each panel. The position of molecular weight markers (kDa) is shown on the left and the estimated size (kDa) of complexes is shown on the right. (b) TOB complex was purified from a strain carrying His-tagged Tob55 and subjected to first dimension BNGE (1^st dim) in two separate lanes of the gel. One lane was transferred to PVDF, and decorated with antibody to Tob55 (top lane in panel). The second lane was removed for second dimension (2^nd dim) electrophoresis by SDS-PAGE as described in the Methods. Following SDS-PAGE, the gel was transferred to nitrocellulose. The membrane was cut into strips corresponding to the molecular weights of Tob55, Tob38, and Tob37 and probed with antibodies to those proteins, respectively (indicated on the left). Sizes of TOB complex following 1^st dimension BNGE are indicated at the top of the panel. (c) As in panel B, except the purification was performed using mitochondria containing His-tagged Tob38 and the SDS-PAGE blot was examined with antibodies to Tob38 and Mdm10. (d) As in panel B except that whole mitochondria were examined for the presence of TOB complexes. (e) As in panel A, except TOB complex was purified from mitochondria isolated from cells expressing only His-tagged versions of different Tob55 isoforms [27]: short Tob55 (Tob55 Short HT), intermediate Tob55 (Tob55 Int HT), or long Tob55 (Tob55 Long HT) as indicated at the top of the panels. Blots were immunodecorated with the antibodies indicated at the bottom of the panels.