Skip to main content
. 2011 Sep;13(9):792–805. doi: 10.1593/neo.11788

Figure 1.

Figure 1

Development of a human esophageal keratinocyte cell line with enhanced Wnt/β-catenin signaling. (A) Map of β-catenin, Lef1, and the CatCLef chimeric construct, as well as the lentiviral vector into which CatCLef is subcloned. (B) Fold change in CatCLef mRNA levels after Lenti-CatCLef infection and selection (STR.L.CatCLef), compared with control lentivirus-infected cells (STR.L), n = 3. (C) Western blot analysis for CatCLef protein levels in control (STR.L) and CatCLef-expressing cell lines (STR.L.CatCLef#1 and #2). (D) Western blot analysis for endogenous β-catenin protein levels after CatCLef expression. (E) Wnt/β-catenin transcriptional activity on a canonical Wnt reporter construct TOPflash in CatCLef-expressing (STR.L.CatCLef) and control cells (STR.L), n = 3.