Figure 5.

CatCLef increases epithelial thickness and cell proliferation when cells cultured under organotypic conditions. Hematoxylin and eosin stain of epithelial tissues sections from STR.L control (A) and STR.L.CatCLef cells (B) after cultured under organotypic conditions. (C) Quantification of epithelial thickness, measured in three regions from three different tissue sections per culture, n = 9. (D and E) Ki67 staining in tissue sections from STR.L (D) and STR.L.CatCLef cells (E). (F) Quantitation of Ki67-positive staining. The number of Ki67+ cells was expressed as a percentage of the total number of basal keratinocytes in a counted field, n = 6.