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. 2011 Sep;13(9):854–863. doi: 10.1593/neo.11594

Figure 1.

Figure 1

FOXO1-derived peptides FO1–6 and FO1-6nls inhibit CDK2-mediated phosphorylation of FOXO1 in vitro. (A) A schematic diagram showing GST-FOXO1 recombinant proteins and the amino acid sequence of the small peptide FO1-6nls that contains the S249 phosphorylation site. FKH indicates forkhead domain; NLS/nls, nuclear localization signal; NES, nuclear export signal; TA, transactivation domain. (B) Reconstituted CDK2/cyclin E kinase assays using FO1–3 plus FO1–6 (left panels) or FO1–2 plus FO1–6 (right panels) as substrates. Mr(KD), relative molecular weight in kilodaltons. (C) Reconstituted CDK2/cyclin E kinase assays using the RB-C protein and FO1–6 as substrates. (D) Reconstituted CDK2/cyclin E kinase assays with substrates of FO1–3 and FO1-6nls. All the experiments were repeated at least two times.