Titers of anti–Sm-p80 antibodies in immunized baboons. Enzyme-linked immunosorbent assay was performed with serum samples from each baboon in their respective control and experimental groups of recombinant protein vaccine formulations (rSm-p80 + CpG ODN or Resiquimod [R848]). Shown are total immunoglobulin G (IgG) (A), IgG1 (B), IgG2 (C), IgM (D), and IgA (E) levels in individual control (HE13, WA84, LA15, and GE35) and vaccinated (ST88, NI31, SE83, and AN25) baboon serum samples collected every 4 weeks. The values represent the mean (± SE) of 3 experiments.