Figure 2.

PGC transformation in EG cells. The process is schematically subdivided in to three main steps: rapid 1 day reprogramming processes involving TSA-induced epigenetic modifications and resetting of various gene and intracellular signalling pathways, a mid-time (2–4 days) establishment of a LIF/STAT3-dependent self-renewal circuit, and the formation of EG cell colonies requiring 7–10 days of culture. Various growth factors and compounds controlling each steps are reported; a cell monolayer producing KL and other not identified compounds is also represented.