Fig. (2).

Activation of ATF6α and XBP1 in response to diclofenac and indometacin. A: Protein extracts from control, thapsigargin- (TG), diclofenac- (DIC) and indomethacine- (IND) treated Huh7 cells for the times indicated in hours (h) were analyzed by SDS-PAGE and Western blot. To detect the low level of endogenous ATF6α, 50 mg of protein extract were loaded on the SDS-PAGE. Arrows indicate uncleaved 90 kDa- and cleaved 50 kDa-ATF6α. B: Total RNA samples extracted from Huh7 cells exposed for the times indicated in minutes (min) to either diclofenac (DIC) or indomethacin (IND) were analyzed by RT-PCR to detect the XBP1 mRNA splicing forms. The migration on the gel of the 452 bps un-spliced fragment of the XBP1 mRNA (XBP1 u) and of the spliced 426 bp fragment (XBP1 s) is indicated.