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Effects of TGF-β1 and cGMP on colocalization of Smad3 and β2-tubulin in PASMC. Serum-starved PASMC were pretreated with cGMP (0.5 mm) or vehicle for 1 h and then exposed to TGF-β1 (2 ng/ml) for 1 h. The cells were fixed and subjected to double immunostaining with anti-Smad3 (red) and anti-β2-tubulin (green) antibodies. Nuclei were stained with DAPI. After merging, colocalization is presented in yellow.
