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. 2011 Aug 25;25(10):1794–1803. doi: 10.1210/me.2011-1009

Table 1.

MS analysis of proteins of interest on 2D-DIGE gel

Spot ID Protein name Accession no. MW (Da) PI Peptide counts Protein score Protein score % C.I. Fold increase (cGMP + TGF-β1 vs. TGF-β1)
14 Tubulin β-2 C chain gi 40018568 49769 4.79 21 184 100 3.33
27 BRCC36 gi 198278575 32994.7 5.84 10 99 100 2.42
15 Vimentin, isoform-β gi 149021114 53668.1 5.06 29 256 100 2.24
13 Vimentin, isoform-β gi 149021114 53668.1 5.06 25 256 100 2.21
17 Vimentin, isoform-β gi 149021114 53668.1 5.06 29 290 100 2.13
18 Aldose reductase giI6978491 35774.3 6 6 41 43 2.0

MW, Molecular weight; PI, isoelectric point. β2-Tubulin is a cytoskeletal protein. BRCC36 and vimentin are both cytosolic and nuclear proteins (24, 25). The low protein score (43% C.I.) of aldose reductase suggests a possibility of incorrect protein identification. ID, Identification.