Table 1.
Spot ID | Protein name | Accession no. | MW (Da) | PI | Peptide counts | Protein score | Protein score % C.I. | Fold increase (cGMP + TGF-β1 vs. TGF-β1) |
---|---|---|---|---|---|---|---|---|
14 | Tubulin β-2 C chain | gi 40018568 | 49769 | 4.79 | 21 | 184 | 100 | 3.33 |
27 | BRCC36 | gi 198278575 | 32994.7 | 5.84 | 10 | 99 | 100 | 2.42 |
15 | Vimentin, isoform-β | gi 149021114 | 53668.1 | 5.06 | 29 | 256 | 100 | 2.24 |
13 | Vimentin, isoform-β | gi 149021114 | 53668.1 | 5.06 | 25 | 256 | 100 | 2.21 |
17 | Vimentin, isoform-β | gi 149021114 | 53668.1 | 5.06 | 29 | 290 | 100 | 2.13 |
18 | Aldose reductase | giI6978491 | 35774.3 | 6 | 6 | 41 | 43 | 2.0 |
MW, Molecular weight; PI, isoelectric point. β2-Tubulin is a cytoskeletal protein. BRCC36 and vimentin are both cytosolic and nuclear proteins (24, 25). The low protein score (43% C.I.) of aldose reductase suggests a possibility of incorrect protein identification. ID, Identification.