Skip to main content
. 2011 Aug 25;25(10):1689–1698. doi: 10.1210/me.2011-1072

Fig. 3.

Fig. 3.

RIP140 and MED1 compete for association with TRα. A, In vitro competition assay of RIP140 and MED1 with regards to binding to TRα. In vitro pull down assay between GST-TRα and 35S-RIP140 WT, with titration of unlabeled MED1 in vitro transcribed and translated (TNT) protein (upper). In vitro pull down assay between GST-TRα and 35S-MED1, with titration of unlabeled RIP140 WT TNT protein (lower). B, In vitro pull down assay between GST-TRα and 35S-RIP140 CN, with titration of unlabeled MED1 TNT protein (upper). In vitro pull down assay between GST-TRα and 35S-RIP140 CP, with titration of unlabeled MED1 TNT protein (lower).