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Acetyl mimetic RIP140 associates with repressive cofactors in vivo. Co-IP of acetyl mimetic Flag-RIP140 CP (K158/287Q) and acetyl negative Flag-RIP140 CN (K158/287A) with endogenous CDK8, and HDAC3 in undifferentiated 3T3-L1 cells in presence of T3 (1 μm, 12 h). CP, Constitutive positive; CN, constitutive negative; CMV, cytomegalovirus promoter driven empty vector; IB, immunoblot.
