Fig. 6.
Gene-expression changes in granulosa cells from adult control and Ctgf cKO (Amhr2cre/+) ovaries after PMSG stimulation. Adult control and Ctgf cKO (Amhr2cre/+) female mice (age 6–8 months) were stimulated with PMSG for 46 h. Granulosa cells were then collected from five independent control and cKO ovaries. Total RNA was isolated and converted to cDNA. Real-time qPCR analysis of granulosa cell mRNA was performed using gene-specific primers. The data are shown as means ± sem. Significant changes in gene expression are seen for Ccnd1 (A), Lhcgr (B), Cyp17a1 (C), Hsd3b (D), Hsd17b7 (E), Ptgfr (F), Sfrp4 (G), Kitl (H), and Myc (I). Other genes examined are shown in Table 4. The control values were set to equal 1. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (vs. control female mice). RQ, Relative quantity.