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. 2011 Oct;138(4):393–419. doi: 10.1085/jgp.201110682

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© 2011 DiFranco and Vergara

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Figure 3. — Effects of external Na on di-8-ANEPPS transients. (A–C) Di-8-ANEPPS transients elicited by depolarizing pulses of 30, 40, 50, 70, 100, and 140 mV (black, red, blue, green, orange, and purple traces, respectively). The red boxes above each optical trace indicate the period during which measurements of steady-state ΔF/F (for Fig. 4 A) were made. (D–F) Di-8-ANEPPS transients elicited by hyperpolarizing pulses of −30, −40, −50, −60, −70, −80, and −90 mV (all traces in black). The arrows indicate the presence of undershoots in the optical records. (G–I) Current records for depolarizing pulses used in A–C, respectively (same color code as in A–C). The current records obtained with hyperpolarizing pulses are not shown. The data in A, D, and G were obtained in 1/2Na external solutions. The data in B, E, and H were obtained in 1/2Na + TTX. The data in C, F, and I were obtained in TEA-Cl external solution + TTX. Fiber parameters: radius, 22 µm; length, 416 µm; capacitance, 5.5 µF/cm².