Skip to main content
. Author manuscript; available in PMC: 2011 Sep 29.
Published in final edited form as: Clin Chem Lab Med. 2007;45(12):1777–1784. doi: 10.1515/CCLM.2007.342

Figure 7.

Figure 7

Western blot analysis showing muscimol-induced expression of GABAA receptor in cells.

Cells (5–7 passages) were plated onto cell culture dishes, grown in complete media and allowed to become 80% confluent. Cells were serum deprived. (A) Mouse brain microvascular endothelial cells (MBMEC) and (C) mouse aorta microvascular endothelial cells (MAMEC) were cultured alone (CT), with 50 μM Hcy, 50 μM muscimol or 50 μM Hcy+50 μM muscimol. After 18 h, equal amounts of cellular protein were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and blotted using GABAA receptor antibody. Corresponding β-actin bands are shown. Accompanying densitometry is shown in (B) and (D), respectively (n=4). *p−0.05 vs. control.