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. Author manuscript; available in PMC: 2011 Sep 29.
Published in final edited form as: Cell Biochem Biophys. 2006;45(2):157–165. doi: 10.1385/CBB:45:2:157

Fig. 3.

Fig. 3

(A) Zymographic analysis of MMP-2 activity in the presence and absence of muscimol: 106 MVEC were cultured alone (E); with 40 μM muscimol (M); with 20 μM Hcy (H); and with Hcy and muscimol or anti-GABA-A antibody (AGA) or baclofen (B). After 24 h cell media were analyzed for MMP-2 activity. Western blot analysis of GABA-A receptor in MVEC. Cells were homogenized. Homogenates were loaded onto reducing SDS-PAGE and blotted using GABA-A receptor antibody. Corresponding actin bands are shown. (B) The bands in zymographic gels and Western blots were scanned and normalized with actin. *p < 0.01 compared with E alone. **p < 0.01 compared with E+H.