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. 2011 Sep 15;4(3):169–178.

Figure 3.

Figure 3

OT treatment enhances OTR mRNA in aorta of group III, a: p<0.001 as compared to group I, b: p<0.001 as compared to group II, c: non significant as compared to group I. (B) PhosphorImager analysis of RT-PCR amplification using specific oligonucleotide primers for OTR mRNA in the aorta of control, adult male rats supplemented with methionine and adult male rats treated with oxytocin. Total RNA isolated from aorta was used for RT-PCR reactions as described in Materials and methods. Lane 1 from the left is the 1-kb ladder control; lane 2: group I, lanes 2: group II, and 3: group III correspond to the primers specific for OTR at 370bp. The results are verified to β Actin mRNAPCR product used as internal standard.