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. Author manuscript; available in PMC: 2011 Sep 29.
Published in final edited form as: J Invest Dermatol. 2011 May 19;131(9):1877–1885. doi: 10.1038/jid.2011.142

Figure 1.

Figure 1

Slug regulates ZEB1 expression. Immunoblots of whole cell lysates (15μg/lane) of transduced melanoma cell lines WM164 (a) and WM9 (b) overexpressing Slug (pLXRN-Slug) or GFP control (pLXRN-GFP). Slug knockdown was achieved by transfection with siRNA against Slug mRNA (siSlug) over a time period of 24 – 72h. Results were compared to unspecific scrambled RNA (scRNA). Samples were separated on a 10% SDS polyacrylamide gel. After transfer to a PVDF membrane, blots were probed with antibodies against Slug and ZEB1. β-Actin was used as a loading control. (c) Analysis of total mRNA extracts of either Slug overexpressing or Slug silenced WM164. Slug, ZEB1 and GAPDH mRNA expression was determined by PCR. E-box binding EMT regulators Snail (d) and Twist (e) were silenced for 24 and 48h using specific siRNAs (siSnail, siTwist). Effects of Snail and Twist silencing on ZEB1 expression were determined by immunoblotting using indicated antibodies. Numbers represent changes in % of controls and normalized to β-Actin or GAPDH.