Table 1. Differential characteristics of strains Halo-G*T, AUS-1 and Naxos II and type strains of related Halorubrum species.
Strains used for comparison: 1, Hrr. coriense DSM 10284T; 2, Hrr. trapanicum JCM 10477T; 3, Hrr. xinjiangense JCM 12388T; 4, Hrr. litoreum JCM 13561T, 5, Hrr. sodomense ATCC 33755T; 6, Hrr. ejinorense CECT 7194T; 7, Hrr. ezzemoulense CECT 7099T. Unless indicated, data for reference strains were taken from McGenity & Grant (2001), Feng et al. (2004), Kharroub et al. (2006), Castillo et al. (2007) and Cui et al. (2007). +, Positive; −, negative; PGS, phosphatidylglycerolsulfate; nd, no data available.
| Feature | Halo-G*T | AUS-1 | Naxos II | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
|---|---|---|---|---|---|---|---|---|---|---|
| Morphology† | r/p | r/p | r/p | lr/p | r/p | sr | r | r/p | r | r/p |
| Cell width (μm) | 0.8 | 0.8 | 0.5–0.8 | 0.5 | 0.7–1.0 | 0.8–1.2 | 0.3–0.5 | 0.5 | 1.0–1.5 | 0.6 |
| Cell length (μm) | 2.0–4.0 | 2.0–4.0 | 2.0–5.0 | 5.0 | 1.5–3.0 | 1.8–2.6 | 2.0–5.0 | 2.5–5.0 | 5.0–8.0 | 1.5–3.0 |
| Pigmentation‡ | rd | rd | rd | rd–o | po | rd | rd | o–rd | rd | rd |
| Motility | + | + | + | + | − | + | + | + | − | + |
| Growth at 2 M NaCl | −/+§ | −/+§ | −/+§ | −/+∥§ | − | + | + | + | − | − |
| Temperature optimum (°C) |
37 | 37 | 37 | 50 | 37 | 40 | 37–42 | 40 | 37 | 37–40 |
| Growth at 10 °C | − | − | − | − | − | + | nd | − | nd | nd |
| Mg2+ requirement | 50 mM | None | 20 mM | 5 mM | nd | None | 30 mM | 5 mM | None | Required |
| Nitrite from nitrate | − | + | − | − | nd | − | + | nd | + | + |
| Hydrolysis of: | ||||||||||
| Starch | + | − | + | +∥ | − | − | − | + | − | − |
| Gelatin | − | − | − | − ∥ | − | nd | − | − | − | − |
| Utilization of: | ||||||||||
| (+)-d-Glucose | + | + | + | + | + | + | + | + | − | + |
| (−)-d-Fructose | + | + | + | + | + | + | − | + | − | − |
| (+)-d-Galactose | + | + | + | +∥ | + | − | + | + | − | − |
| Sucrose | − | − | − | + | + | + | + | − | − | + |
| Lactose | + | + | + | +∥ | − | − | − | + | − | − |
| Maltose | + | + | + | +∥ | + | + | + | + | − | + |
| Presence of: | ||||||||||
| PGS | + | + | + | + | + | + | + | + | − | + |
| S-DGD | + | + | + | + | + | + | + | + | − | + |
| G+C content (mol%) | 65.5 | 65.5 | 66.5 | 67.3∥ | 64.3 | 68.0 | 64.9 | 67.4 | 61.0 | 61.9 |
†
lr, Long rod; p, pleomorphic; r, rod; sr, short rod.
‡
o, Orange; po, pale orange; prd, pale red; rd, red.
§
Very slow growth, after about 4 weeks.
∥
Data from this study.