Semithin sections of SR-BI-EGFP-expressing ldlA7 cells after photoconversion. The overview shows the darkened cells in the center of the exposed area. The majority of these cells show DAB-deposits, indicating a homogeneous signal conversion. On these sections, the quality of the conversion process is assessed, different staining patterns are listed, and areas of interest for further electron microscopic analyses defined (a). The influence of the illumination is shown by the amount of generated DAB-deposits, which increases with the duration of the illumination (b 30 minm, c 60 min). After 60 min, the percentage of cells with extremely intense reactions is increased (c
arrowheads)