Fig. 3.

Activity of in vitro-synthesized MobA protein. Substrate DNA was made by PCR of T2 gene 39 DNA with only one primer labeled at its 5′ end in each reaction. Template DNAs for the in vitro transcription were wild type T4 (wt), a mutant T4 strain with a deletion distant from the mobA gene (saΔ9), and T4 containing a tyrosine-to-amber nonsense mutation at codon 195 of mobA (Y195am) (Fig. 2). Control reactions were either in vitro protein synthesis reactions carried out without addition of template DNA (Mock) or with no protein synthesis reaction added (NP) in the endonuclease assay. Positions of end-labeled DNA size standards (in kb) are on the left.