Table 1.
% l-arabinose |
|||||
Expression plasmid* | Experiment | 0 | 0.00002 | 0.0002 | 0.002 |
pMobA | 1 | 97 | 99 | 94 | 56 |
2 | 88 | 98 | 92 | 54 | |
pF-CphI | 1 | 1 | 0 | 2 | 0 |
2 | 0 | 0 | 1 | 0 | |
pBAD | 1 | 2 | 1 | 2 | 0 |
2 | 0 | 0 | 2 | 1 |
T2 progeny plaques (of 100 picked) that hybridize to the bypass sequence probe MobA25.
*Cells also contain the donor plasmid pT4 (39-60ΔmobA).