Fig. 5.

Engineering of the inhibitory TLR2 lipopeptide ligand - GM1-targeted, linoleate-containing TLR2 ligand (GML). (A) Schematic of GML. (B) Wild-type, TLR2^−/−, or MyD88^−/− mice treated with an intrascrotal injection of 150 μL of saline alone or saline containing the indicated ligands (37.5 ng/g GML and/or 20 ng/g TNFα) either alone or in combination, and evaluated 4.5 h later for the number of emigrated neutrophils in the cremaster tissue. (C and D) Male C57BL/6 mice were given a dorsal s.c. inoculum of live luminescent E. coli in the presence or absence of GML. (C) Image of two examples of these mice (with and without GML) taken at the time of inoculation and 4 h later. (D) Luminescence normalized to the detected luminescence at the time of inoculation, observed over 6 h.