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. 2011 Sep 19;108(39):16307–16312. doi: 10.1073/pnas.1107341108

Fig. 4.

Fig. 4.

FoxO1 is an upstream regulator of VCAM1 gene expression. Quantitative RT-PCR analyses for fold change of the transcript of indicated genes using RNA isolated from FoxO1-WT and -null heart (A) and allantois (B) (n = 3–4). Gene expression in WT heart and allantois was normalized to 1. Note the significant dysregulation of VCAM1 gene expression in FoxO1-null heart and allantois (#P < 0.001 versus WT). (C) Immunohistochemical analyses for Vcam-1 in E8.5 FoxO1-WT (+/+) and -null (−/−) embryos revealed significant attenuation of Vcam-1 levels (arrows) in FoxO1-null heart and allantois (Al) compared with WT littermates. Head and chorion (Ch) are indicated. (D) Schematic of the VCAM1 upstream promoter region (−0.7 kb) fused to a luciferase (Luc) reporter. Coexpression of a constitutively active FoxO1 (caFoxO1) elicited dose-dependent activation of VCAM1 reporter activity harboring WT, but not mutated, FoxO-responsive elements (FREs) (*P < 0.001 versus control; #P < 0.01 versus WT FREs). Error bars represent mean ± SD, and P values were calculated by Student's t test.