Figure 1.
Camui is a FRET biosensor based on the structure of CaMKII. (A) Binding of Ca2+/CaM results in a conformational shift that increases the distance between the N-terminal catalytic domain and the C-terminal regulatory/association domains, reducing FRET between the CFP/YFP pair. (B) Titration of Camui with CaM in the presence of saturating Ca2+ shows the dynamic range of the sensor.