Skip to main content
. Author manuscript; available in PMC: 2012 Sep 16.
Published in final edited form as: Circ Res. 2011 Aug 11;109(7):729–738. doi: 10.1161/CIRCRESAHA.111.247148

Figure 3.

Figure 3

Localization of Camui in cardiomyocytes. (A) Fluorescent confocal microscopy of CFP and YFP emission signals from myocytes expressing Camui. A sample line scan confirms Camui association with myofilaments. (B) A sample ratio plot shows localized differences in Camui activation in the nucleus (Nuc), perinuclear space (Peri) and cytoplasm (Cyto) at baseline conditions. (C) Selective photobleach of the YFP acceptor results in increased donor fluorescence, which is ablated by activation of CaMKII via increased [Ca2+]I. (D) CFP fluorescence is not enhanced by YFP photobleach in a 1:1 stoichiometric manner.