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. Author manuscript; available in PMC: 2011 Sep 29.
Published in final edited form as: Bioorg Med Chem Lett. 2008 Aug 19;18(22):5995–5999. doi: 10.1016/j.bmcl.2008.08.046

Figure 2.

Figure 2

Fluorescent images of Rat-1 fibroblasts simultaneously pulse-labeled with two reactive amino acids. Cells were pulse-labeled for 5 h with 1 mM Met, 1 mM Aha and 1 mM Hpg (1:1), 3 mM Aha and 1 mM Hpg (3:1), or 1 mM Aha and 3 mM Hpg (1:3). Cells were fixed and blocked before dye-labeling with LR-azide and DMAC-alkyne. Images were false-colored in ImageJ. Scale bar represents 20 µm.