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. Author manuscript; available in PMC: 2011 Sep 29.
Published in final edited form as: Sci Signal. 2009 Oct 13;2(92):ra63. doi: 10.1126/scisignal.2000382

Fig. 4. Act1 promotes Lys 63-linked polyubiquitination of TRAF6 at Lys 124.

Fig. 4

(A) Traf6−/− MEFs resconstituted with either vector or TRAF6 (WT) and its mutants (K124R, C70A) were treated with IL-17, followed by Western with indicated antibodies. (B) Traf6−/− MEFs resconstituted with either vector or TRAF6 (WT) and its mutants (K124R, C70A) were subjected to NFκB luciferase assay. (C) and (D) GST-TRAF6 and its mutants (K124R, C70A) were used as substrates in the ubiquitination assays, followed by Western with antibodies against TRAF6 (C) and ubiquitin (D). [The results of real-time PCR and ELISA are calculated from five independent experiments. Group differences were calculated by t test * P < 0.05.]